Composite

Part:BBa_K3329067:Design

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyTag003*3-Scl2*2-sfGFP for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1564
    Illegal NgoMIV site found at 2076
    Illegal NgoMIV site found at 2277
    Illegal AgeI site found at 535
    Illegal AgeI site found at 1411
    Illegal AgeI site found at 1420
    Illegal AgeI site found at 1492
    Illegal AgeI site found at 1546
    Illegal AgeI site found at 1762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 151
    Illegal BsaI site found at 907
    Illegal BsaI site found at 1963
    Illegal BsaI.rc site found at 532


Design Notes

Optimized for E. coli


Source

From Article///

References